|
|
|
| Registros recuperados: 233 | |
|
|
| Maia,Clara Yohana; Soares,Nazaré Suziane; Castro,Ana Cecília Ribeiro; Queirós,João Ravelly Alves de; Aragão,Fernando Antonio Souza de; Bordallo,Patricia do Nascimento. |
| ABSTRACT Anthurium affine is an ornamental Brazilian native plant very appreciated due to its long-lasting durability. To explore the genetic diversity available, 21 A. affine half-sibling progeny were evaluated using morphoagronomic and molecular descriptors. The morphoagronomic descriptors used were: plant height, plant diameter, growth habit, leaf number, leaf blade length, leaf blade width, petiole length, petiole diameter, leaf margin undulation, general appearance; soil cover rate and Random Amplified Polymorphic DNA (RAPD) markers the molecular one. All the descriptors within the progeny showed variability and general appearance, leaf margin undulation and soil cover rate are the ones that most contribute to the plants divergence. From 45 RAPD... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clustering; Characterization; Genetic variation; Ornamental; RAPD. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400410 |
| |
|
| |
|
|
| Diniz,Leandro Eugênio Cardamoni; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Torres,Fabrício Medeiros; Ruas,Eduardo Augusto; Santos,Melissa de Oliveira; Sera,Tumoru; Ruas,Paulo Maurício. |
| The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea arabica; Genetic variability; RAPD; Restriction endonucleases. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500002 |
| |
|
| |
|
| |
|
|
| Silva,Rosana Bellan de Oliveira; Fusco-Almeida,Ana Marisa; Matsumoto,Marcelo Teruyuki; Baeza,Lilian Cristiane; Benaducci,Tatiane; Mendes-Giannini,Maria José Soares. |
| Trichosporon asahii is an opportunistic pathogen, associated with a high mortality rate in immunocompromised patients. In this study, ten isolates, recovered from oral cavity and urine of patients in Intensive Care Units (ICU) over six months, were identified by classical and molecular methods, typed by RAPD and tested in vitro for susceptibility to fluconazole, itraconazole, 5-flucytosine and amphotericin B. A total agreement between the identification of Trichosporon sp by PCR based on sequences of the Internal Transcribed Spacer Regions (ITS) and on the sequences of small-subunit (SSU) ribosomal DNA (rDNA) was found. Randomly amplified of polymorphic DNA (RAPD), with primers P6 and M13, was used to determine the genomic profiles. The dendogram analysis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trichosporon; PCR; RAPD; Susceptibility; Antifungal drugs. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300033 |
| |
|
| |
|
|
| Mori,Edson S; Martins,Dagoberto; Velini,Edivaldo D; Marino,Celso L; Gouvêa,Cantídio F; Leite,Suzi M.M; Camacho,Edwin; Guries,Raymond P. |
| The aquatic plant species Egeria densa (Planch.) Casp and E. najas Planchon occur naturally in the Paraná River Basin of southeastern Brazil. Hydroelectric dam construction in the river basin has created several lakes, changing the ecology of the river and altering the population ecology of Egeria. Large, dense populations of Egeria now grow in Jupiá Lake and its tributaries, occasionally blocking hydroelectric turbines. This study is part of a larger project examining environmental changes associated with large dam projects; the research objective of this study was to assess patterns of genetic diversity in Egeria populations growing in Jupiá and Três Irmãos Lakes and their Paraná River tributaries using genetic markers. Forty-two plants of E. najas and... |
| Tipo: Journal article |
Palavras-chave: Egeria; Dam; Isoenzyme; Genetic distance; RAPD; Reservoir. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000200008 |
| |
|
| |
|
|
| Valera-Montero,LL; Muñoz-Rodríguez,PJ; Silos-Espino,H; Flores-Benítez,S. |
| Guava fruit produced in Calvillo, Aguascalientes (Mexico) is considered to be of the best quality in this country. Nevertheless, growers from this place empirically know that there is a noticeable variation among individual trees within the same orchard, and variation among individuals from different orchards. In order to have a clear evidence of this, morphology analysis of guava was performed taking data from seventy nine individuals out of thirty six orchards, while RAPD was performed on a subset of twenty six individuals. Similarity was found for morphology data ranging from 87-100%, while similarity from RAPD data ranged from 30-100%. Combined data of RAPD and morphology showed similarity greater than 80%. Clades from combined data sorted genotypes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Morphological analysis; RAPD; Variation; Guava. |
| Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200002 |
| |
|
|
| Sofia,Silvia H.; Galindo,Bruno A.; Paula,Francine M.; Sodré,Leda M.K.; Martinez,Cláudia B.R.. |
| In this study, random amplified polymorphic DNA (RAPD) markers were applied to analyze the genetic diversity of samples of the Neotropical catfish Hypostomus ancistroides, collected from four sites (S1, S2, S3 and S4) along an urban stream in Southern Brazil. The 11 primers used in RAPD analysis amplified 147 loci, 76 (51.7%) of which were polymorphic. The proportions of polymorphic loci observed in the four samples were: 29.93% (S1), 31.97% (S2), 23.81% (S3) and 38.77% (S4). The average heterozygosity within sampling localities ranged from 0.1230 to 0.1526 and unbiased genetic distances ranged from 0.0253 to 0.0445. AMOVA partitioned 90.85% of the total variation within samples and 9.15% among samples. Excepting for the sample pair S1-S2 (phiST = 0.02784;... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Loricariid fish; Genetic diversity; RAPD; Siluriformes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200027 |
| |
|
| |
|
| |
|
| |
|
|
| Sera,Tumoru; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Diniz,Leandro Eugênio Cardamone; Carvalho,Valdemar de Paula; Rampim,Leandro; Ruas,Eduardo Augusto; Silveira,Sheila Recepute da. |
| Knowledge of the genetic variability among genotypes is important for the transfer of useful genes and to maximize the use of available germplasm resources. This study was carried out to assess the genetic variability of 14 elite Coffea arabica cultivars using random amplified polymorphic DNA (RAPD) associated with a prior digestion of genomic DNA with restriction endonucleases. The accessions were obtained from the Coffea collection maintained at the Instituto Agronômico do Paraná (IAPAR), located in Londrina, Paraná, Brazil. Twenty-four informative RAPD primers, used in association with restriction enzymes, yielded 330 reproducible and scorable DNA bands, of which 224 (68%) were polymorphic. The amplified products were used to estimate the genetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea arabica; RAPD; Restriction endonucleases. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100010 |
| |
|
|
| GOMEZ,PATRICIA I; GONZALEZ,MARIELA A. |
| Eight Chilean strains of Dunaliella salina obtained within a restricted geographic range, but exhibiting a high variability in their morphology, rate of growth and carotenogenic capacity, were analyzed by Random Amplified Polymorphic DNA (RAPD-PCR). Twenty of the 50 random primers (D, P, OPA and OPD series) that were tested amplified reproducible bands and were useful for comparative analysis of the strains. Of 107 polymorphic genetic markers, 49 were strain-specific. A great genetic variability was found among the strains in spite of their geographic proximity. In addition, phenetic analysis of the data showed close agreement between the morpho-physiological attributes and the genetic diversity of the strains |
| Tipo: Journal article |
Palavras-chave: SS-carotene; Dunaliella salina; Genetic polymorphism; RAPD. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100012 |
| |
|
|
| REYES,EUGENIO; BARAHONA,SALVADOR; FISCHMAN,OLGA; NIKLITSCHEK,MAURICIO; BAEZA,MARCELO; CIFUENTES,VÍCTOR. |
| In this work 20 clinical and 3 environmental yeast isolates were characterized by classical morphological and physiological methods, as well as molecular methods based on PCR of the ITS1-5.8S rDNA-ITS2 region. The characteristic morphology and biochemical profiles observed in these samples correspond to those described for the Pichia genera, more specifically to P. anomala. The profiles of susceptibility to five antifungal drugs were determined by two broth dilution methods. The results obtained by both methods were comparable and showed that clinical isolates presented more resistance to azoles, amphotericin B, and 5-fluorocytosine, than environmental ones did. By amplification and sequencing of internal transcribed spacers (ITS1 and ITS2) and the... |
| Tipo: Journal article |
Palavras-chave: Pichia anomala; 5.8S rDNA; RAPD; Internal transcribed spacer; Antifungal drugs. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500004 |
| |
|
|
| Almeida,Margarete Teresa Gottardo; Rubio,Fernando Gôngora; Garcia,Doroti Oliveira; Pavarino-Bertelli,Érika Cristina; Rossit,Andrea Regina Baptista; Bando,Silvia Yano; Silbert,Suzane; Goloni-Bertollo,Eny Maria; Soares,Márcia Maria Costa Nunes; Martinez,Marina Baquerizo. |
| Stenotrophomonas maltophilia is a Gram-negative bacillus, which is becoming widely recognized as an important nosocomial pathogen. The main objective of this study was to evaluate the genetic relatedness, by random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of 86 clinical isolates of S. maltophilia (colonization 22, infection 64) obtained from 79 hospitalized patients, from different geographic regions of São Paulo State. The genotypic analysis performed by RAPD and PFGE was used in 24 isolates for genetic identity confirmation. The results were congruent between the two methods but it was not possible to link genetic profiles with the studied variables, clinical state and geographic area, probably due to the great... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stenotrophomonas maltophilia; RAPD; PFGE. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200017 |
| |
|
|
| SANTANNA,JULIANE R; MIYAMOTO,CLÁUDIA T; ROSADA,LÚCIA J; FRANCO,CLAUDINÉIA C S; KANESHIMA,EDILSON N; CASTRO-PRADO,MARIALBA A A. |
| The genetic variation among nine soybean-originating isolates of Colletotrichum truncatum from different Brazilian states was studied. Nitrate non-utilizing (nit) mutants were obtained with potassium chlorate and used to characterize vegetative compatibility reactions, heterokaryosis and RAPD profile. Based on pairings of nit mutants from the different isolates, five vegetative complementation groups (VCG) were identified, and barriers to the formation of heterokaryons were observed among isolates derived from the same geographic area. No complementation was observed among any of the nit mutants recovered from the isolate A, which was designed heterokaryon-self-incompatible. Based on RAPD analysis, a polymorphism was detected among the wild isolate C and... |
| Tipo: Journal article |
Palavras-chave: Genetic variability; Heterokaryosis; Parasexual recombination; RAPD. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100007 |
| |
|
|
| Grazziotin,Felipe; Echeverrigaray,Sergio. |
| The genetic relationships among 11 species of Bothrops found in Brazil were determined using random amplified fragment (RAPD) information. A total of 239 amplified bands were scored using 20 aleatory primers. Most of the bands (88.7%) were polymorphic. Phylogenetic analysis of the data determined three similarity groups within Bothrops: Group I- B. alternatus, B. neuwiedi, B. cotiara, and B. jararacusu; Group II- B. insularis, B. jararaca, and B. erythromelas; and Group III- B. moojeni, B. leucurus, and B. atrox. These groups coincided with those obtained by other methods, indicating that RAPD's could be a useful tool for the evaluation of genetic relationships at the interspecific level. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops; RAPD; Molecular markers; Genetic relationship. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300005 |
| |
| Registros recuperados: 233 | |
|
|
|
